Development of a slide latex agglutination test for rotavirus antigen detection.

Development of a Latex Agglutination Method for Diagnosis of Rotavirus Infections

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect...

Frequency of Rotavirus Infection in Children with Acute Gastroenteritis in Jahrom, South of Iran

Background: Rotavirus is a major cause of acute diarrhea in children worldwide. It is an important cause of death among young children in developing countries. The aim of this study was to evaluate the prevalence of rotavirus infection in children less than five years old hospitalized for gastroenteritis in Jahrom. Methods: One hundred and two stool samples were collected from children less tha...

Development of a simple and rapid latex test for rotavirus in stool samples.

In the fields of infectious diseases, a variety of immunoassay methods has been reported. More simple, rapid and cost-effective manual tests for infectious diseases are needed. For the purpose of simple diagnosis of rotavirus infection, a rapid test against human rotavirus was developed. As for the preparation of the reagent, clinically isolated human rotavirus was cultured using MA-104 cells. ...